The emergence of m obile c olistin r esistance (mcr) threatens to undermine the clinical efficacy of the last antibiotic that can be used to treat serious infections caused by Gram-negative pathogens.Here we measure the fitness cost of a newly discovered MCR-3 using in vitro growth and competition assays. mcr-3 expression confers a lower fitness cost than mcr-1, as determined by competitive

Direct mobilization of mobilizable plasmids into 91 indigenous bacteria within mixed natural communities has been detected before (Hill et al., 1992; van 92 Elsas et al., 1998), but was never directly quantified. 93 94 Here, we present a novel experimental method to estimate the plasmid mobilization potential of a 95 mixed bacterial community, using IncQ RSF1010 as model plasmid.

Host range of mobilization of the IncQ plasmid pIE723 and self-transfer of plasmid pIPO2 from P. fluorescens R2f (chr::Tn5) to different rifampin-resistant recipient strains - "Isolation, Characterization, and Transfer of Cryptic Gene-Mobilizing Plasmids in the Wheat Rhizosphere" (2014). Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Front Microbiol 5. DOI: 10.3389/fmicb.2014.00730 IV Klümper U, Dechesne A, Riber L, Gülay A, Brandt KK, Sørensen SJ, Smets BF. (2015). Taxon specific modulation of soil community permissiveness towards broad host range plasmid pKJK5 under metal Plasmids are important vehicles for rapid adaptation of bacterial populations to changing environmental conditions. It is thought that to reduce the cost of plasmid carriage, only a fraction of a local population carries plasmids or is permissive to plasmid uptake.

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Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Uli eKlümper, Ariadni eDroumpali, Arnaud eDechesne and Barth F Smets No static citation data No static citation data Cite 2010-05-28 · Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Klümper U, Droumpali A, Dechesne A, Smets BF. Front Microbiol, 5:730, 22 Dec 2014 Cited by: 8 articles | PMID: 25566238 | PMCID: PMC4273639. Free to read & use Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Overview of attention for article published in Frontiers in Microbiology, December 2014 Altmetric Badge Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Uli Klümper, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets Download PDF (3 MB) Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Microbial Physiology and Metabolism. Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Uli Klümper, Ariadni Droumpali, Arnaud Dechesne & Barth F. Smets; Frontiers in Long-term manure exposure increases soil bacterial community potential for plasmid uptake. Environ Microbiol Rep, 6(2), 125-130.

MODELLING - Mathematical & biology models, alternatives to animals testing 2 Ulcer Therapy Gene transfer in skin equivalents and stem cells: novel development and retain the potential to turn into the different types of cells of innovation, or help building future Community research policies. Microbiology & Mol.

By Bahl, Barth, Boe-Hansen, Buchanan-Wollaston, Dechesne, Dubey, Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Barth Smets , Arnaud Dechesne , and Uli Klümper An individual-based approach to explain plasmid invasion in bacterial populations Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community.

By Bahl, Barth, Boe-Hansen, Buchanan-Wollaston, Dechesne, Dubey, Gelder, Grahn, Gregory, Grohmann, Götz, Haase, Heuer, Heuer, Heuer, Hill, Hirt, Honda, Klümper

By Arnaud Dechesne. 2021-01-08 1998-03-01 2016-09-23 2018-11-23 Moreover, conjugation via plasmid transfer is postulated to be prevalent in complex microbial communities (e.g., the gut and soil microbiomes) due to the high local density, diversity, and abundance of strains/species along with mobile genetic elements (Ogilvie et al, 2012). 2021-03-25 Dive into the research topics where Ariadni Droumpali is active. These topic labels come from the works of this person.

Therefore, the fate of the plasmid in the microbial population will be In in vitro competition assays, plasmid-carrying and plasmid-free clones are mixed From the initial and final density of each clone, it is possible to calculat We discuss the potentials and limitations of methods used to determine the host transfer plays an important role not only in the mobilization and dissemination of technology to a mixed bacterial population to reliably associate pl 7 Jul 2020 The effect of genetic dominance on bacterial evolution has generally Many MGEs, including plasmids and filamentous phages, replicate frequency of antibiotic resistant mutants in the bacterial population at To pe 27 Jan 2018 The conjugative plasmid, pLS20, isolated from Bacillus subtilis natto, has an to develop a novel genetic tool for the rapid, easy, and repetitive mobilization of up to 113 kb of chromosomal DNA containing oriTLS20, 22 Mar 2018 Where possible the potency assay should include a measure of the functional activity of the therapeutic sequence or the product of it. This  7 Jul 2019 plant domestication on the microbial communities inhabiting the rhizosphere. and in-situ experiments were conducted to assess the potential of O015 Novel genomic determinants of respiratory metabolism in extremophil 25 Oct 2019 RSF1010-based plasmids replicate in diverse bacterial strains but produce genetically tractable strains from mixed wild cultures. In addition to their potential as production platforms, cyanobacteria it harbors 12 Dec 2018 Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Front. Microbiol.
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Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Uli Klümper * , Ariadni Droumpali , Arnaud Dechesne and Barth F. Smets * … Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Uli Klümper*, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets* Department of Environmental Engineering,Technical University of Denmark, Kongens Lyngby, Denmark Edited by: Kornelia Smalla, Julius Kühn-Institut, Germany Reviewed by: Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. a novel framework and experimental method to estimate the mobilization potential of mixed communities. Direct mobilization of mobilizable plasmids into 91 indigenous bacteria within mixed natural communities has been detected before (Hill et al., 1992; van 92 Elsas et al., 1998), but was never directly quantified. 93 94 Here, we present a novel experimental method to estimate the plasmid mobilization potential of a 95 mixed bacterial community, using IncQ RSF1010 as model plasmid. Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities.

Large plasmids were transferred to clinical isolates via either conjugation or direct electroporation when possible. For transfer by conjugation, the TOP10 strain (Life Technologies, USA) was used as donor strain due to its high competence and leucine auxotrophy. 2016-09-23 · By using plasmid-encoded TEM β-lactamase mediated ampicillin resistances as a proof of principle system, we (1) developed a fluorescence in situ hybridization-test (FISH) capable to detect the respective mRNAs, (2) implemented an immunofluorescence test to identify the corresponding proteins and (3) compared these two microscopic tests with an established colorimetric nitrocefin assay to 2019-08-28 · 39 plasmids, members within complex microbial communities might have a hitherto unrecognized 40 potential to maintain plasmids for long-term community-wide access. 41 available under aCC-BY-NC-ND 4.0 International license.
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2016-09-23 · However, many rapid molecular methods like PCR only detect the sole presence of a potential resistance gene, do not provide information regarding efficient transcription, expression and functionality and, in addition, cannot assign resistance genes to species level in mixed cultures. By using plasmid-encoded TEM β-lactamase mediated ampicillin

Front. Microbiol., 5 (2014), p. 730. View Record in Scopus Google Scholar. Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Front Microbiol 5 : 730. PubMed PubMed Central Google Scholar Klümper U, Droumpali A, Dechesne A, Smets BF. Novel assay to measure the plasmid mobilizing potential of mixed microbial communities.